DNA Barcoding a Complete Matrix of Stereoisomeric Small Molecules

DNA Barcoding: a new tool with wide array of applications

DNA barcoding is a new term introduced in to scientific literatures by Hebert and coworkers almost a decade ago. The concept of barcoding alone is well-known to the public: a series of black bars printed on many commercial products (Universal Product Code), which are used to distinguish different products. Advances made in molecular biology and molecular techniques late 20th century e.g. sequen...

Phasing of single DNA molecules by massively parallel barcoding

High-throughput sequencing platforms mainly produce short-read data, resulting in a loss of phasing information for many of the genetic variants analysed. For certain applications, it is vital to know which variant alleles are connected to each individual DNA molecule. Here we demonstrate a method for massively parallel barcoding and phasing of single DNA molecules. First, a primer library with...

INTERACTION OF PROTEINS WITH SMALL MOLECULES

Molecular recognition of DNA by small molecules.

Chemists, like artists, are able to construct new threedimensional objects, molecules and materials that exist only in the mind of a person. I became interested in creating novel molecular shapes with properties different from those found in nature shortly after arriving at Caltech in 1973. One cannot design without the brushes and paint of the craft. Indeed, modern organic chemists, standing o...

Allosteric modulation of DNA by small molecules.

Many human diseases are caused by dysregulated gene expression. The oversupply of transcription factors may be required for the growth and metastatic behavior of human cancers. Cell permeable small molecules that can be programmed to disrupt transcription factor-DNA interfaces could silence aberrant gene expression pathways. Pyrrole-imidazole polyamides are DNA minor-groove binding molecules th...